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KMID : 0613820060160061014
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Journal of Life Science
2006 Volume.16 No. 6 p.1014 ~ p.1028
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The Effects of EGEE on Lectins Binding Pattern of Rat Cerebral Cortex in Developmental Phase
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Lee Eung-Hee
Jo Un-Bock
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Abstract
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This study attempted to investigate the developmental alterations of the cerebral cortex. The effects of EGEE on the developmental cerebral cortex in the prenatal, postnatal and adults were examined by lectin histochemical methods. To investigate the change of sugar residues of glycoconjugates, biotinylated lectins(DBA, SBA, PNA, BSL-1, RCA-1, sWGA, UEA-1, Con A and LCA) were detected with by IHC using ABC kit. In the case of injection of EGEE, the reactions to Con A and LCA were shown in binding phase in the cerebral cortex commonly, and the reactions to PNA, RCA-1 and LCA were shown partially, the number of lectins to be shown reaction were decreased, and there were no reactions to DBA, SBA, BSL-1, RCA-1 and UEA-1. The reaction to Con A was similar to control group during developmental phases. The reaction to LCA was increased in the fetal, suckling, and weanning phases compared with control group. But there were no reactions to SBA and sWGA, the reaction to PNA was decreased in the frontal and occipital cortex and no reaction to sWGA in the fetal phase. There were no reactions to sWGA and PNA in the suckling phase and, no reaction to PNA and sWGA. The reaction to Con A was decreased in the frontal, parietal and occipital cortexes and, the reaction to LCA was decreased in the frontal and occipital cortexes in adult phase. As the results, the effects of EGEE, environmental hormone on the each part of cerebral cortex have shown differences. But, It had deep effect on the differentiation and growth in the cerebral cortex pathologically. In particular, the effect was severe in suckling phase. Galactosyl-(β-1,3)-N-acetyl-Dgalactosamine, β-N-acetyl-D-galactosamine and β-N-acetyl-D-glucosamine were decreased while α-D-mannose and α-D-glucose were increased. It affected the sugar metabloism, and it was severe in fetal and suckling phases.
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KEYWORD
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EGEE, lectin, glycoconjugates, developmental cerebral cortex
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